Contact an Account ManagerDetermination of the aminoglycoside antibiotics sisomicin and netilmicin in dried blood spots on filter discs by HPLC with precolumn derivatization and fluorimetric detection
Summary
Type: HPLC
Application #: 103410
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: fluorescence, excitation 340nm, emission 450nm
Conditions: eluent: methanol - counter ion solution (2.5g Na 1-heptanesulphonate and 42ml acetic acid in 208ml dist. water), 80:20 (v/v) for sisomicin, 75:25 (v/v) for netilmicin
flow rate 0.9ml/min
Authors: Tawa R
Source: J Chromatogr 490 (1989) 125 - 132
Details
Legend: Fig. 4. HPLC of the OPA-b-MP derivatives of (a) sisomicin in the DBS and the
blank and (b) netilmicin in the DBS and the blank. Concentrations of sisomicin
and netilmicin, 20 and 50µg/ml of whole bl